Retinoblastoma 1 antibody (C-Term)

Catalog No. ABIN2854790

The Rabbit Polyclonal anti-Retinoblastoma 1 antibody has been validated for WB, IF, IP, IHC (p) and ICC. It is suitable to detect Retinoblastoma 1 in samples from Human.

Quick Overview for Retinoblastoma 1 antibody (C-Term) (ABIN2854790)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Rhesus Monkey

Cross-Reactivity (Details): Rhesus Monkey (100 %)

Characteristics: Rabbit Polyclonal antibody to Rb (retinoblastoma 1)
Rb antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Rb. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: A431 , *CL1-0 , *CL1-5 ,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb

Background: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Cellular Localization: Nucleus

Molecular Weight: 106 kDa

Gene ID: 5925

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy