CD44 antibody

Catalog No. ABIN2855292

Product Details anti-CD44 Antibody

Target: CD44

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Live Cell Imaging (LCI)

Specificity: IP/MS validation was supported by references (PMID:30377381)

Cross-Reactivity: Human, Rabbit, Rat

Characteristics: Rabbit Polyclonal antibody to CD44 (CD44 Molecule (Indian blood group))
CD44 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD44. The exact sequence is proprietary.

Isotype: IgG

Product Specific Information:

What can the CD44 antibody ABIN2855292 be used for? This polyclonal, unconjugated rabbit anti-CD44 antibody is excellent for detection of the surface protein CD44 in western blot, immunocytochemistry, immunofluorescence and immunohistochemistry. It has the reactivities mouse, rabbit, human. Recombinant protein comprising a sequence within the central region of the human CD44 molecule was used as the immunogen.

What validation data is available for this CD44 antibody? The CD44 antibody has been referenced in 3 publications to date, which are given below and can be viewed at Pubmed. There are 8 images of the rabbit anti-CD44 antibody showing its performance in Western blot, IHC and ICC / IF. Use our CD44 antibody for reliable detection of CD44.

What is the function of CD44? CD44 is a cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. CD44 molecule participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. (UniProt)

Application Details

Application Notes: WB: 1:1000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MDA-MB-231(24 μg/ml Tunicamycin treatment for 16 hr) , HeLa(12 μg/ml Tunicamycin treatment for 48 hr)

Validation: Comparison, IP/MS, KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CD44 antibody (ABIN2855292)

Li, Gao, Wang, Song, Xu, Xie, Zhou, Pan, Peng, Zhang, Ge, Zhong: "Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations." in: Frontiers in molecular neuroscience, Vol. 12, pp. 212, (2019) (PubMed).

Sun, Wu, Sheu, Chang, Chen, Lin, Chuang, Hsu, Chang: "Integrin β3 and CD44 levels determine the effects of the OPN-a splicing variant on lung cancer cell growth." in: Oncotarget, Vol. 7, Issue 34, pp. 55572-55584, (2018) (PubMed).

Jiang, Guo, Li, Yu, Zhou, Gong: "Combination Twist1 and CA15-3 in axillary lymph nodes for breast cancer prognosis." in: Molecular medicine reports, Vol. 15, Issue 3, pp. 1123-1134, (2017) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44 molecule (Indian blood group) (CD44 Products)

Background: The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Cellular Localization: Membrane

Molecular Weight: 82 kDa

Gene ID: 960

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling