CDK4 antibody

Catalog No. ABIN2855529

This anti-CDK4 antibody is a Rabbit Polyclonal antibody detecting CDK4 in WB, IF, ICC and IHC (p). Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CDK4 antibody (ABIN2855529)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-CDK4 Antibody

Cross-Reactivity: Cow, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to CDK4 (cyclin-dependent kinase 4)
CDK4 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CDK4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: PC-12 , 293T

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.82 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CDK4 antibody (ABIN2855529)

Chen, Huang, Lin, Fang: "Piperlongumine induces autophagy in biliary cancer cells via reactive oxygen species-activated Erk signaling pathway." in: International journal of molecular medicine, Vol. 44, Issue 5, pp. 1687-1696, (2020) (PubMed).

Li, Du, Aldana-Masangkay, Wang, Urak, Forman, Rosen, Chen: "Regulation of miR-34b/c-targeted gene expression program by SUMOylation." in: Nucleic acids research, Vol. 46, Issue 14, pp. 7108-7123, (2019) (PubMed).

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: cyclin dependent kinase 4

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Molecular Weight: 34 kDa

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size