CYP24A1 antibody (Center)

Catalog No. ABIN2856211

Product Details anti-CYP24A1 Antibody

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP24A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): mouse (87 %), Rat (88 %)

Characteristics: Rabbit Polyclonal antibody to CYP24A1 (cytochrome P450, family 24, subfamily A, polypeptide 1)
CYP24A1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CYP24A1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CYP24A1

Target: CYP24A1 (Cytochrome P450, Family 24, Subfamily A, Polypeptide 1 (CYP24A1))

Alternative Name: CYP24A1 (CYP24A1 Products)

Synonyms: CP24 antibody, CYP24 antibody, HCAI antibody, P450-CC24 antibody, 24-OHase antibody, Cyp24 antibody, 25-hydroxyvitaminD324-hydroxylase antibody, VITD12 antibody, CP450CC24 antibody, cytochrome P450 family 24 subfamily A member 1 antibody, cytochrome P450, family 24, subfamily a, polypeptide 1 antibody, cytochrome P450, family 24, subfamily A, polypeptide 1 antibody, CYP24A1 antibody, Cyp24a1 antibody

Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Mitochondrion

Molecular Weight: 59 kDa

Gene ID: 1591

Pathways: Metabolism of Steroid Hormones and Vitamin D