Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

POLR3A antibody (N-Term)

POLR3A Reactivity: Human WB, ICC, IF, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2856286
  • Target See all POLR3A Antibodies
    POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
    Binding Specificity
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 26
    • 16
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 26
    Rabbit
    Clonality
    • 26
    Polyclonal
    Conjugate
    • 15
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLR3A antibody is un-conjugated
    Application
    • 14
    • 10
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Cross-Reactivity
    Mouse (Murine), Chicken, Xenopus laevis, Cow (Bovine), Xenopus tropicalis
    Cross-Reactivity (Details)
    Mouse (97 %), Chicken (97 %), Xenopus laevis (94 %), Bovine (98 %), Xenopus tropicalis (94 %)
    Characteristics
    Rabbit Polyclonal antibody to POLR3A (polymerase (RNA) III (DNA directed) polypeptide A, 155 kDa)
    POLR3A antibody
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the N-terminus region of human POLR3A. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
    Comment

    Positive Control: HCT116 , SK-N-SH , IMR32 , SK-N-AS

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.49 mg/mL
    Buffer
    1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target
    POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
    Alternative Name
    POLR3A (POLR3A Products)
    Synonyms
    9330175N20Rik antibody, BC053071 antibody, RPC1 antibody, RPC155 antibody, RGD1305574 antibody, ADDH antibody, HLD7 antibody, hRPC155 antibody, polymerase (RNA) III (DNA directed) polypeptide A antibody, RNA polymerase III subunit A antibody, Polr3a antibody, POLR3A antibody
    Background
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

    Cellular Localization: Nucleus
    Molecular Weight
    156 kDa
    Gene ID
    11128
You are here:
Support