VPS11 antibody (Center)

Catalog No. ABIN2856524

The Rabbit Polyclonal anti-VPS11 antibody has been validated for WB, IF, ICC and IHC (p). It is suitable to detect VPS11 in samples from Human.

Quick Overview for VPS11 antibody (Center) (ABIN2856524)

Target: VPS11 (Vacuolar Protein Sorting 11 Homolog (VPS11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS11 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-VPS11 Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio), Medaka, Xenopus tropicalis, Cow (Bovine)

Cross-Reactivity (Details): Mouse (100 %), Rat (100 %), Zebrafish (85 %), Japanese Medaka (100 %), Xenopus Tropicalis (92 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to VPS11 (vacuolar protein sorting 11 homolog (S. cerevisiae))
VPS11 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VPS11. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for VPS11

Target: VPS11 (Vacuolar Protein Sorting 11 Homolog (VPS11))

Alternative Name: VPS11

Background: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Cellular Localization: Late endosome membrane, Peripheral membrane protein, Cytoplasmic side , Lysosome membrane, Peripheral membrane protein, Cytoplasmic side

Molecular Weight: 108 kDa

Gene ID: 55823

Pathways: SARS-CoV-2 Protein Interactome