HLA-DMB antibody (C-Term)

Catalog No. ABIN2856610

This Rabbit Polyclonal antibody specifically detects HLA-DMB in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for HLA-DMB antibody (C-Term) (ABIN2856610)

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HLA-DMB Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to HLA-DMB (major histocompatibility complex, class II, DM beta)
HLA-DMB antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HLA-DMB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HLA-DMB transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HLA-DMB

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Alternative Name: major histocompatibility complex, class II, DM beta

Background: HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Cellular Localization: Late endosome membrane , Lysosome membrane

Molecular Weight: 29 kDa

Gene ID: 3109

UniProt: P28068

Pathways: Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response