VEROTOXIN (Subunit A) antibody
Reactivity: E. coli
EIA, Neut
Host: Mouse
Monoclonal
557
unconjugated
-
- Target
- VEROTOXIN
- Binding Specificity
- Subunit A
- Reactivity
- E. coli
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
-
Application
- Enzyme Immunoassay (EIA), Neutralization (Neut)
- Purification
- Protein A affinity chromatography
- Purity
- > 90 % pure
- Clone
- 557
- Isotype
- IgG2
-
-
- Application Notes
-
ELISA. Neutralization.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 0.1 mg/mL ( OD280nm, E0.1% = 1.4)
- Buffer
- 0.01 M PBS, pH 7.2, containing 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Upon receipt, aliquot and store at -20 °C.
-
- Target
- VEROTOXIN
- Background
- Verotoxins are produced by certain enterohaemorrhagic strains of Escherichia coli. Serotype O157:H7 has been the strain most commonly associated with large food bourne outbreaks and has been implicated in a wide spectrum of diseases including blood diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome. The main sources of infection are contaminated foods of animal origin such as meat and dairy products. Verotoxins, also called Shiga like toxins( SLT) because of their similarity to toxins produced by Shigella dysenteriae, are classified into two main categories: verotoxin 1 (VT1, SLT1, Stx1) and 2 (VT2, SLT2, Stx2). Verotoxin production is the most common criteria for the detection of this group of bacteria rather than tests for the cytopathic effect on Vero cells that gave the toxins their name. Verotoxins contain a single A subunit and multiple copies of a B subunit. The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits.Synonyms: SLT-2 A subunit, SLT-2a, SLT-IIa, Verocytotoxin 2 subunit A, Verotoxin 2 subunit A, rRNA N-glycosidase 2, stx2A, stxA2
- Gene ID
- 10730
- UniProt
- P09385
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