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GFP antibody (HRP)

This Mouse Monoclonal antibody specifically detects GFP in WB, ELISA and IHC. It exhibits reactivity toward Aequorea victoria.
Catalog No. ABIN289132

Quick Overview for GFP antibody (HRP) (ABIN289132)

Target

See all GFP Antibodies
GFP (Green Fluorescent Protein (GFP))

Reactivity

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Aequorea victoria

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This GFP antibody is conjugated to HRP

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone

215
  • Specificity

    The monoclonal detects wild type and recombinant GFP. This antibody does notdetect enhanced GFP.

    Immunogen

    GFP antibody (HRP) was raised in Mouse using The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. as the immunogen.

    Isotype

    IgG2a
  • Application Notes

    ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000-1:5,000
    Optimal conditions should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Concentration

    Lot specific

    Buffer

    Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % thimerisol. Immunoglobulin and protease free.

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze/thaw cycles.
    Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Dilute only prior to immediate use.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
  • Target

    GFP (Green Fluorescent Protein (GFP))

    Alternative Name

    GFP
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