IL-1 beta antibody

Catalog No. ABIN289475

The Rabbit Polyclonal anti-IL-1 beta antibody is suitable to detect IL-1 beta in samples from Rat. It has been validated for WB, ELISA and InhibAb.

Quick Overview for IL-1 beta antibody (ABIN289475)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (InhibAb)

Product Details anti-IL-1 beta Antibody

Purification: Purified

Immunogen: IL1 beta antibody was raised in rabbit using highly pure recombinant rat IL-1-beta as the immunogen.

Application Details

Application Notes: ELISA: 0.5-2 µg/mL, Inhibition: 0.09-0.14 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of > 0.2 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from 0.5 x PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1 beta

Background: IL1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1a and IL1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL2 release, B-cell maturation and proliferation, mitogenic FGFlike activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells.

Molecular Weight: 17.3 kDa (predicted detection band MW)

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome