TNF alpha antibody

Catalog No. ABIN289560

Cited in 3+ publications. The Rabbit Polyclonal anti-TNF alpha antibody (ABIN289560) specifically detects TNF alpha in WB, ELISA and InhibAb. The antibody is reactive with Mouse samples.

Quick Overview for TNF alpha antibody (ABIN289560)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (InhibAb)

Product Details anti-TNF alpha Antibody

Purification: Purified

Immunogen: TNF alpha antibody was raised in rabbit using highly pure recombinant murine TNF-alpha as the immunogen.

Application Details

Application Notes: ELISA: 0.5-2 µg/mL, Inhibition: 0.04-0.05 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration 0.1 - 1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

References for anti-TNF alpha antibody (ABIN289560)

McCullough, McMullen, Poulsen, Kim, Medof, Nagy: "Anaphylatoxin Receptors C3aR and C5aR1 Are Important Factors That Influence the Impact of Ethanol on the Adipose Secretome." in: Frontiers in immunology, Vol. 9, pp. 2133, (2019) (PubMed).

McCullough, McMullen, Das, Roychowdhury, Strainic, Medof, Nagy: "Differential contribution of complement receptor C5aR in myeloid and non-myeloid cells in chronic ethanol-induced liver injury in mice." in: Molecular immunology, Vol. 75, pp. 122-32, (2017) (PubMed).

Zhuge, Cederbaum: "Inhibition of the mitochondrial permeability transition by cyclosporin A prevents pyrazole plus lipopolysaccharide-induced liver injury in mice." in: Free radical biology & medicine, Vol. 46, Issue 3, pp. 406-13, (2009) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.

Molecular Weight: 17.3 kDa (predicted detection band MW)

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome