Histone H2B antibody (AA 111-125)

Catalog No. ABIN291581

The Rabbit Polyclonal anti-Histone H2B antibody is suitable to detect Histone H2B in samples from Human, Mouse, Rat, Cow, Chicken, Zebrafish (Danio rerio), Insect and Drosophila melanogaster. It has been validated for WB, IHC and IHC (p).

Quick Overview for Histone H2B antibody (AA 111-125) (ABIN291581)

Target: Histone H2B

Reactivity: Human, Mouse, Rat, Cow, Chicken, Zebrafish (Danio rerio), Insect, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H2B Antibody

Binding Specificity: AA 111-125

Specificity: A 15 kD band is observed. This is routinely quality controlled in human lysates.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).

Purification: Protein G purified

Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B, GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Histone H2B

Target: Histone H2B