GPR101 antibody (Cytoplasmic Domain)

Catalog No. ABIN291950

Product Details anti-GPR101 Antibody

Target: GPR101 (G Protein-Coupled Receptor 101 (GPR101))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR101 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human GPR101. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZBTB10 (47 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (95%) Bovine, Horse, Pig (89%) Bat, Panda, Rabbit (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR101. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (95%), Bovine, Horse, Pig (89%), Bat, Panda, Rabbit (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (7.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 7.3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR101

Target: GPR101 (G Protein-Coupled Receptor 101 (GPR101))

Alternative Name: GPCR6 / GPR101 (GPR101 Products)

Synonyms: GPR101 antibody, GPCR6 antibody, Gm365 antibody, RGD1564196 antibody, G protein-coupled receptor 101 antibody, GPR101 antibody, Gpr101 antibody

Background: Name/Gene ID: GPR101
Subfamily: Orphan-U
Family: GPCR

Synonyms: GPR101, G protein-coupled receptor 101, GPCR6

Gene ID: 83550