JNK2 antibody (AA 373-389)

Catalog No. ABIN293095

This Rabbit Polyclonal antibody specifically detects JNK2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Pig, Rabbit, Cow and Bat.

Quick Overview for JNK2 antibody (AA 373-389) (ABIN293095)

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Reactivity: Human, Pig, Rabbit, Cow, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JNK2 Antibody

Binding Specificity: AA 373-389

Specificity: A synthetic peptide corresponding to amino acids 373-389 (KDQPSDAAVSSNATPSQ) of human JNK2 was used as immunogen. This amino acid sequence is 100 % homologous in cow and dog, and 94 % in mouse and rat.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Elephant, Bat, Bovine, Rabbit, Pig (100%) Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Horse (94%) Platypus (82%).

Purification: Protein G purified

Immunogen: A synthetic peptide corresponding to amino acids 373-389 (KDQPSDAAVSSNATPSQ) of human JNK2, GenBank no. gi|21237736|ref|NP_002743.3|. This amino sequence is 100% homologous in cow and dog, and 94% in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Elephant, Bat, Bovine, Rabbit, Pig (100%), Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Horse (94%), Platypus (82%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for JNK2

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Alternative Name: MAPK9 / JNK2 / SAPK

Background: Name/Gene ID: MAPK9
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK9, C-Jun kinase 2, C-Jun N-terminal kinase 2, JNK2B, JNK2, JNK2ALPHA, MAP kinase 9, p54a, JNK2BETA, PRKM9, SAPK, MAPK 9, p54aSAPK, SAPK1a, JNK-55, JNK2A, Jun kinase

Gene ID: 5601

NCBI Accession: NP_002743

UniProt: P45984

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins