WNT2B antibody
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- Target See all WNT2B Antibodies
- WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This WNT2B antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Specificity
- Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.
- Purification
- Affinity purified
- Immunogen
- E. coli-derived rmWnt-2b.
- Isotype
- IgG
- Top Product
- Discover our top product WNT2B Primary Antibody
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- Application Notes
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Wnt-2b. The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows approximately 20 % cross-reactivity with rhWnt-2, and less than 5 % cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Wnt-2b in BT20 human breast carcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights-557-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Wnt-2b. The detection limit for rmWnt-2b is approximately 0.5 ng/well. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- Lyophilized.
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- Target
- WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))
- Alternative Name
- WNT2B (WNT2B Products)
- Synonyms
- WNT13 antibody, XWNT2 antibody, wnt2b antibody, XWnt-2 antibody, Xwnt-2b antibody, wnt-2b antibody, xwnt2b antibody, WNT2B antibody, Wnt13 antibody, Wnt family member 2B antibody, wingless-type MMTV integration site family, member 2Ba antibody, Wnt family member 2B L homeolog antibody, wingless-type MMTV integration site family, member 2B antibody, wingless-type MMTV integration site family, member 2Bb antibody, WNT2B antibody, Wnt2b antibody, wnt2ba antibody, wnt2b.L antibody, wnt2bb antibody
- Background
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Name/Gene ID: WNT2B
Family: WNT
Synonyms: WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of - Gene ID
- 7482
- UniProt
- Q93097
- Pathways
- WNT Signaling
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