GFP antibody

Catalog No. ABIN294467

The Chicken Polyclonal anti-GFP antibody has been validated for WB and ICC. It is suitable to detect GFP in samples from Aequorea victoria.

Quick Overview for GFP antibody (ABIN294467)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-GFP Antibody

Specificity: Recognizes green fluorescent protein (GFP), Mr 30kD and GFP-fusion proteins.

Purification: Ammonium sulfate precipitation

Immunogen: His-tagged green fluorescent protein of Aequorea victoria.

Isotype: IgY

Application Details

Application Notes: Approved: ICC, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP* and a chemiluminescence detection system. Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding GFP.

Comment:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212