c-FOS antibody (AA 4-17)

Catalog No. ABIN295405

Product Details anti-c-FOS Antibody

Target: c-FOS (c-Fos) (c-Fos)

Binding Specificity: AA 4-17

Reactivity: Human, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This c-FOS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes human c-fos. Species cross-reactivity: Rat.

Purification: Purified

Immunogen: Synthetic c-fos peptide (residues Ser Gly Phe Asn Ala Asp Tyr Glu Ala Ser Ser Ser Arg Cys) corresponding to amino acids 4-17 of human Fos, conjugated to bovine thyroglobulin.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IF, IHC, IHC-Fr (1:500 - 1:4000), IHC-P (1:500 - 1:4000), WB (10 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 10 μg/mL Detects a band at ~39kD. Immunohistochemistry: 1:500-1:4000 (4?C, 1-3 days) Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Recommended fixation is 4 % PFA with 0.5 % picric acid (Li et al 1999). Triton X-100 pretreatment in the block only. Antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 500 μL sterile ddH20

Concentration: Lot specific

Buffer: Lyophilized. No preservatives.

Preservative: Without preservative

Handling Advice: Avoid frequent freeze thaws. Store undiluted.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Alternative Name: FOS / C-FOS (c-Fos Products)

Synonyms: AP-1 antibody, C-FOS antibody, p55 antibody, c-fos antibody, D12Rfj1 antibody, cFos antibody, cFOS antibody, fos antibody, Fos proto-oncogene, AP-1 transcription factor subunit antibody, FBJ osteosarcoma oncogene antibody, proto-oncogene c-Fos-like antibody, FOS antibody, Fos antibody, LOC101078177 antibody

Background: Name/Gene ID: FOS

Synonyms: FOS, Activator protein 1, AP-1, Cellular oncogene c-fos, Cellular oncogene fos, Proto-oncogene c-Fos, v-fos, C-FOS, G0s7, p55

Gene ID: 2353

Pathways: S100 Proteins