MAPK7 antibody (pThr218, Tyr220)
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- Target See all MAPK7 Antibodies
- MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))
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Binding Specificity
- AA 213-224, pThr218, Tyr220
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Reactivity
- Human
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This MAPK7 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Recognizes human phospho-BMK1/Erk5 (Thr218, Tyr220), Mr ~110kD. Predicted to cross-react with mouse and rat based on sequence homology.
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 (HQYFM[pT]E[pY]VATR), KLH-conjugated. The immunizing sequence is identical in mouse, 9/12aa identical in rat.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product MAPK7 Primary Antibody
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- Application Notes
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Approved: WB
Usage: Suitable for use in Western Blot. Western Blot: 1-:250-1:500 detects phospho-BMK1/Erk5 (Thr218, Tyr220) in RIPA lysates from HeLa cells treated with sorbitol. Lysates from untreated or sorbitol-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:500) or anti-BMK1/Erk5 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (BMK1/Erk5 or phospho- BMK1/Erk5 (110kD).
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 100 mM Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))
- Alternative Name
- MAPK7 / ERK5 (MAPK7 Products)
- Synonyms
- BMK1 antibody, ERK4 antibody, ERK5 antibody, PRKM7 antibody, Erk5-T antibody, ATMPK7 antibody, F8D23.5 antibody, F8D23_5 antibody, MAP kinase 7 antibody, Bmk1 antibody, Erk5 antibody, Prkm7 antibody, ERK-5 antibody, bmk1 antibody, erk4 antibody, mapk7 antibody, prkm7 antibody, mitogen-activated protein kinase 7 antibody, MAP kinase 7 antibody, mitogen-activated protein kinase 7 L homeolog antibody, MAPK7 antibody, Mapk7 antibody, MPK7 antibody, mapk7.L antibody
- Background
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Name/Gene ID: MAPK7
Subfamily: MAPK
Family: Protein Kinase
Synonyms: MAPK7, Big MAP kinase 1, BMK-1, Erk4, ERK5, MAPK 7, PRKM7, BMK1, BMK1 kinase, ERK-5, MAP kinase 7 - Gene ID
- 5598
- UniProt
- Q13164
- Pathways
- MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling
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