GFP antibody (HRP)

Catalog No. ABIN297131

The Goat Polyclonal anti-GFP antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect GFP in samples from Aequorea victoria.

Quick Overview for GFP antibody (HRP) (ABIN297131)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GFP Antibody (HRP)

Specificity: Recognizes wild type, recombinant and enhanced GFP. Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against human, mouse and rat serum proteins.

Purification: Immunoaffinity purified

Immunogen: GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IF, IHC (1:500 - 1:3000), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:10000-1:50000. Sandwich or capture. Recognizes wild type, recombinant and enhanced forms of GFP. Immunohistochemistry: 1:500-1:3000. Immunofluorescence: Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic. (E. coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Western Blot: 1:1000-1:5000. Detects a 42kD band. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212