AKT 1/2/3 antibody (pTyr312)

Catalog No. ABIN3019995

Product Details anti-AKT 1/2/3 Antibody

Target: AKT 1/2/3

Binding Specificity: pTyr312

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT 1/2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Phosphorylated Antibodies

Purification: Affinity purification

Immunogen: A phospho specific peptide corresponding to residues surrounding Y315 of human AKT1

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:1000,IHC,1:50 - 1:100,IF,1:100 - 1:200

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-AKT 1/2/3 antibody (ABIN3019995)

Bai, Zhao, Zhu, Zhou, Zhao, Zhang, Guo, Lu: "LZ205, a newly synthesized flavonoid compound, exerts anti-inflammatory effect by inhibiting M1 macrophage polarization through regulating PI3K/AKT/mTOR signaling pathway." in: Experimental cell research, Vol. 364, Issue 1, pp. 84-94, (2018) (PubMed).

Dinesh, Rasool et al.: "Berberine inhibits IL-21/IL-21R mediated inflammatory proliferation of fibroblast-like synoviocytes through the attenuation of PI3K/Akt signaling pathway and ameliorates IL-21 mediated ..." in: Cytokine, Vol. 106, pp. 54-66, (2018) (PubMed).

Li, Fan, Yang, Zhao, Cao: "The coordinated effects of Apatinib and Tripterine on the proliferation, invasiveness and apoptosis of human hepatoma Hep3B cells." in: Oncology letters, Vol. 16, Issue 1, pp. 353-361, (2018) (PubMed).

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1/AKT2/AKT3 (AKT 1/2/3 Products)

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011],AKT1/AKT2/AKT3,Protein phosphorylation,Protein phosphorylation,Protein phosphorylation,AKT1/AKT2/AKT3

Molecular Weight: 48 kDa/55 kDa/51 kDa/54 kDa

Gene ID: 207, 208, 10000

UniProt: P31749, P31751, Q9Y243