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AKT 1/2/3 antibody (pTyr312)

Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3019995
  • Target See all AKT 1/2/3 products
    AKT 1/2/3
    Binding Specificity
    • 24
    • 22
    • 22
    • 22
    • 19
    • 19
    • 18
    • 16
    • 16
    • 16
    • 7
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr312
    Reactivity
    • 147
    • 135
    • 121
    • 44
    • 31
    • 26
    • 23
    • 15
    • 7
    • 6
    • 4
    • 4
    Human
    Host
    • 145
    • 2
    Rabbit
    Clonality
    • 134
    • 13
    Polyclonal
    Conjugate
    • 41
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AKT 1/2/3 antibody is un-conjugated
    Application
    • 133
    • 79
    • 78
    • 77
    • 33
    • 30
    • 29
    • 18
    • 9
    • 7
    • 6
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Cross-Reactivity
    Human, Mouse, Rat
    Characteristics
    Phosphorylated Antibodies
    Purification
    Affinity purification
    Immunogen
    A phospho specific peptide corresponding to residues surrounding Y315 of human AKT1
    Isotype
    IgG
  • Application Notes
    WB,1:500 - 1:1000,IHC,1:50 - 1:100,IF,1:100 - 1:200
    Restrictions
    For Research Use only
  • Buffer
    PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Avoid freeze / thaw cycles.
  • Bai, Zhao, Zhu, Zhou, Zhao, Zhang, Guo, Lu: "LZ205, a newly synthesized flavonoid compound, exerts anti-inflammatory effect by inhibiting M1 macrophage polarization through regulating PI3K/AKT/mTOR signaling pathway." in: Experimental cell research, Vol. 364, Issue 1, pp. 84-94, (2018) (PubMed).

    Dinesh, Rasool et al.: "Berberine inhibits IL-21/IL-21R mediated inflammatory proliferation of fibroblast-like synoviocytes through the attenuation of PI3K/Akt signaling pathway and ameliorates IL-21 mediated ..." in: Cytokine, Vol. 106, pp. 54-66, (2018) (PubMed).

    Li, Fan, Yang, Zhao, Cao: "The coordinated effects of Apatinib and Tripterine on the proliferation, invasiveness and apoptosis of human hepatoma Hep3B cells." in: Oncology letters, Vol. 16, Issue 1, pp. 353-361, (2018) (PubMed).

  • Target
    AKT 1/2/3
    Alternative Name
    AKT1/AKT2/AKT3 (AKT 1/2/3 Products)
    Background
    The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011],AKT1/AKT2/AKT3,Protein phosphorylation,Protein phosphorylation,Protein phosphorylation,AKT1/AKT2/AKT3
    Molecular Weight
    48 kDa/55 kDa/51 kDa/54 kDa
    Gene ID
    207, 208, 10000
    UniProt
    P31749, P31751, Q9Y243
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