CHRM3 antibody (Cytoplasmic Domain)

Catalog No. ABIN302340

Product Details anti-CHRM3 Antibody

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects Muscarinic Acetylcholine Receptor M3 (CHRM3).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: CHRM3 antibody was raised against synthetic peptide from 3rd cytoplasmic domain of human CHRM3.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Alternative Name: Muscarinic Acetylcholine Receptor M3 (CHRM3 Products)

Synonyms: EGBRS antibody, HM3 antibody, MACHR antibody, Chrm-3 antibody, M3 antibody, M3R antibody, cholinergic receptor muscarinic 3 antibody, cholinergic receptor, muscarinic 3 antibody, cholinergic receptor, muscarinic 3, cardiac antibody, CHRM3 antibody, Chrm3 antibody

Background: Muscarinic acetylcholine receptor 3 is an Acetylcholine Receptor that controls smooth muscle contraction by mediating phosphoinositide hydrolysis and Ca2+ mobilization. Activation of this receptor causes secretion in glandular tissues. Mouse studies suggest that the M3 receptor may play a role in appetite stimulation and food intake.Synonyms: CHRM3

Gene ID: 1131

NCBI Accession: NP_000731

UniProt: P20309

Pathways: Synaptic Membrane