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CDKN1B antibody

This Mouse Monoclonal antibody specifically detects CDKN1B in WB, IF, FACS and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.
Catalog No. ABIN3023777

Quick Overview for CDKN1B antibody (ABIN3023777)

Target

See all CDKN1B Antibodies
CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivity

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Human, Mouse, Rat, Monkey

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CDKN1B antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

KIP1-769
  • Characteristics

    This mAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

    Purification

    Protein G affinity chromatography

    Immunogen

    Recombinant human protein was used as the immunogen for the p27Kip1 antibody.

    Isotype

    IgG1 kappa
  • Application Notes

    Optimal dilution of the p27Kip1 antibody to be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions

    For Research Use only
  • Concentration

    0.2 mg/mL

    Buffer

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

    Alternative Name

    p27Kip1

    Background

    This mAb recognizes a 27  kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

    Pathways

    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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