Nuclear Antigen antibody
-
- Target
- Nuclear Antigen
-
Reactivity
- Human, Rat, Mouse
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
-
Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Characteristics
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
- Purification
- Protein G affinity chromatography
- Immunogen
- Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
- Clone
- NUCM1-1
- Isotype
- IgG1 kappa
-
-
- Application Notes
-
Titering of the Nuclear Antigen antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
- Target
- Nuclear Antigen
- Background
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
-