Recombinant Keratin Acidic (AE1) antibody

Catalog No. ABIN7861617

The Mouse Monoclonal anti-Keratin Acidic antibody is suitable to detect Keratin Acidic in samples from Human, Rat, Chicken, Cow, Mouse, Rabbit, Monkey, Dog and Turtle. It has been validated for IHC and WB.

Quick Overview for Recombinant Keratin Acidic (AE1) antibody (ABIN7861617)

Target: Keratin Acidic (AE1)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Chicken, Cow, Mouse, Rabbit, Monkey, Dog, Turtle

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RKRTL-8751

Product Details

Purpose: Recombinant Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody

Specificity: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human epidermal keratin

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details

Target: Keratin Acidic (AE1)

Alternative Name: Cytokeratin, Acidic

Background:

K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Skin or squamous cell carcinoma (IHC). A431.

Molecular Weight: 40-56.5kDa

Gene ID: 374454, 334989