Thyroglobulin antibody

Catalog No. ABIN3023946

Product Details anti-Thyroglobulin Antibody

Target: Thyroglobulin (TG)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Human thyroid follicular cells were used as the immunogen for this Thyroglobulin antibody.

Clone: 2H11-6E1 or TGB04-TGB05

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Thyroglobulin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: Thyroglobulin (TG Products)

Synonyms: TG antibody, cb717 antibody, AITD3 antibody, TGN antibody, Tgn antibody, cog antibody, thyroglobulin antibody, TG antibody, tg antibody, LOC701526 antibody, LOC701756 antibody, LOC100434297 antibody, Tg antibody

Background: Thyroglobulin is a 660  kDa dimeric pre-protein with mutiple glycosylation sites, detected at ~300  kDa in western blot. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for thyroglobulin antibody even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently thyroglobulin negative. Adenocarcinomas of nonthyroid origin do not react with this antibody cocktail. It is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the origin of the tumor. Thyroglobulin + calcitonin antibody, can identify medullary carcinomas of the thyroid. Furthermore, thyroglobulin + TTF1 antibody can be a reliable marker to differentiate between primary thyroid and lung neoplasms.

Gene ID: 7038

Pathways: Thyroid Hormone Synthesis