NEFH antibody
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- Target See all NEFH Antibodies
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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Reactivity
- Human, Rat, Mouse, Chicken, Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NEFH antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Characteristics
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This antibody reacts with a 200 kDa protein, identified as heavy subunit of neurofilaments, or NF-H. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H), plus alpha internexin or peripherin. Each neurofilament subunit contains a globular head, an alpha helical rod domain, and variable tail domains that differ in length and amino acid content.
Neurofilament antibody stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for neurofilament antibody. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
- Purification
- Protein G affinity chromatography
- Immunogen
- The triton-X 100 insoluble protein fraction of rat brain was used as the immunogen for this Neurofilament Heavy antibody.
- Clone
- RT97
- Isotype
- IgG1
- Top Product
- Discover our top product NEFH Primary Antibody
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the Neurofilament Heavy antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
- Alternative Name
- Neurofilament Heavy (NEFH Products)
- Synonyms
- NF-H antibody, NF200 antibody, Nfh antibody, mKIAA0845 antibody, NFH antibody, NEFH antibody, neurofilament heavy polypeptide antibody, neurofilament, heavy polypeptide antibody, neurofilament heavy antibody, MCYG_06231 antibody, Nefh antibody, NEFH antibody
- Background
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This antibody reacts with a 200 kDa protein, identified as heavy subunit of neurofilaments, or NF-H. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H), plus alpha internexin or peripherin. Each neurofilament subunit contains a globular head, an alpha helical rod domain, and variable tail domains that differ in length and amino acid content.
Neurofilament antibody stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for neurofilament antibody. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
- Gene ID
- 4744
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