CLIP antibody (AA 25-39)

Catalog No. ABIN3024112

Product Details anti-CLIP Antibody

Target: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Binding Specificity: AA 25-39

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLIP antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA

Purification: Protein G affinity chromatography

Immunogen: Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone were used as the immunogen for this ACTH antibody.

Clone: ADCT1-1

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM Citrate Buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. ELISA: order BSA free format for coating,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE):0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CLIP

Target: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Alternative Name: ACTH / CLIP (CLIP Products)

Background: ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Gene ID: 5443