GZMB antibody (AA 73-187)

Catalog No. ABIN3024894

The Rabbit Polyclonal anti-GZMB antibody has been validated for WB, FACS, IF and IHC (p). It is suitable to detect GZMB in samples from Human.

Quick Overview for GZMB antibody (AA 73-187) (ABIN3024894)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GZMB Antibody

Binding Specificity: AA 73-187

Purification: Protein A affinity chromatography

Immunogen: A recombinant fragment (amino acids 73-187) from the human protein was used as the immunogen for the Granzyme B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Granzyme B antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Granzyme B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: Granzyme B

Background: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'non-self' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.

Pathways: Apoptosis, Caspase Cascade in Apoptosis