NGFR antibody (Extracellular)

Catalog No. ABIN3025048

The Mouse Monoclonal anti-NGFR antibody (Clone NGFR5) (ABIN3025048) specifically detects NGFR in IHC (p). The antibody is reactive with Human samples.

Quick Overview for NGFR antibody (Extracellular) (ABIN3025048)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NGFR5

Product Details anti-NGFR Antibody

Binding Specificity: AA 1-160, Extracellular

Purpose: p75NTR Antibody

Purification: Protein G affinity chromatography

Immunogen: NGFR from A875 melanoma cells was used as the immunogen for the p75NTR antibody. Its epitope has been localized to amino acids 1-160 of the extracellular domain of NGFR/NTR.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the p75NTR antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the p75NTR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: p75NTR

Background: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding