MAML2 antibody

Catalog No. ABIN3025218

Product Details anti-MAML2 Antibody

Target: MAML2 (Mastermind-Like 2 (MAML2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAML2 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: A human recombinant protein was used as the immunogen for this MAML2 antibody.

Clone: MMLP2-1

Isotype: IgG2a

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML2 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the MAML2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MAML2

Target: MAML2 (Mastermind-Like 2 (MAML2))

Alternative Name: MAML2 (MAML2 Products)

Synonyms: 5930431H10 antibody, BC032967 antibody, MAM-3 antibody, MAM2 antibody, MAM3 antibody, MLL-MAML2 antibody, mastermind like 2 (Drosophila) antibody, mastermind-like transcriptional coactivator 2 antibody, mastermind like transcriptional coactivator 2 antibody, Maml2 antibody, MAML2 antibody

Background: Mastermind-like 2, also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, it interacts with the ankyrin repeats of the Notch proteins (1-4). This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.

Gene ID: 84441