NAPSA antibody

Catalog No. ABIN3025326

Product Details anti-NAPSA Antibody

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAPSA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment from the human protein was used as the immunogen for the Napsin A antibody.

Clone: NAPSA-1238

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Napsin A antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Napsin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Alternative Name: Napsin A (NAPSA Products)

Synonyms: KAP antibody, Kdap antibody, NAP1 antibody, NAPA antibody, SNAPA antibody, pronapsin antibody, kap antibody, kdap antibody, nap1 antibody, napa antibody, napsa antibody, snapa antibody, napsin A aspartic peptidase antibody, napsin A aspartic peptidase L homeolog antibody, NAPSA antibody, Napsa antibody, napsa.L antibody

Background: Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis