PCNA antibody

Catalog No. ABIN3025663

This anti-PCNA antibody is a Mouse Monoclonal antibody detecting PCNA in WB, IF, FACS and IHC (p). Suitable for Human, Mouse, Rat, Chicken, Monkey, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio) and Drosophila melanogaster.

Quick Overview for PCNA antibody (ABIN3025663)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio), Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM350

Product Details anti-PCNA Antibody

Purification: Protein G affinity chromatography

Immunogen: Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: Recognizes a non-histone protein of 36  kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy