BAX antibody (AA 3-16)

Catalog No. ABIN3025801

The Mouse Monoclonal anti-BAX antibody (Clone SPM336) (ABIN3025801) specifically detects BAX in WB, IF, FACS and IHC (p). The antibody is reactive with Human samples.

Quick Overview for BAX antibody (AA 3-16) (ABIN3025801)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: SPM336

Product Details anti-BAX Antibody

Binding Specificity: AA 3-16

Purpose: Anti-Bax Antibody (azide and preservative free)

Sequence: GSGEQPRGGG PTSS

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Bax antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Background: Recognizes a protein of 21 kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response