CD99 antibody

Catalog No. ABIN3025987

Product Details anti-CD99 Antibody

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

No Cross-Reactivity: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the CD99 antibody.

Clone: MIC2-877

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the CD99 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD99

Target: CD99

Alternative Name: CD99 (CD99 Products)

Synonyms: 1110061M03Rik antibody, 2410026K10Rik antibody, D4 antibody, Pilr-l antibody, pilr-1 antibody, HBA71 antibody, MIC2 antibody, MIC2X antibody, MIC2Y antibody, MSK5X antibody, CD99 antigen antibody, CD99 molecule (Xg blood group) antibody, CD99 molecule antibody, Cd99 antibody, CD99 antibody

Target Type: Viral Protein

Background: Recognizes a sialoglycoprotein of 27-32  kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32  kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. It is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.