Insulin Receptor antibody (Chain beta, Internal Region)

Catalog No. ABIN302612

The Rabbit Polyclonal anti-Insulin Receptor antibody has been validated for IHC (p). It is suitable to detect Insulin Receptor in samples from Human.

Quick Overview for Insulin Receptor antibody (Chain beta, Internal Region) (ABIN302612)

Target: Insulin Receptor (INSR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Insulin Receptor Antibody

Binding Specificity: Chain beta, Internal Region

Specificity: This antibody detects Insulin Receptor (INSR) at internal domain.

Purification: Immunoaffinity Chromatography

Immunogen: INSR antibody was raised against synthetic peptide from internal domain of human INSR / Insulin Receptor.

Application Details

Application Notes: Immunohistochemistry on paraffin section (10 μg/mL)Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undlited at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: CD220 / INSR

Background: INSR is an insulin receptor type protein kinase. Binding of insulin to INSR stimulates glucose uptake. Two insulin receptor mRNA transcripts resulting from alternative splicing of exon 11 in the receptor gene are expressed in a highly regulated tissue-specific fashion. Mutations in the INSR gene contribute to diseases, including of various forms of insulin resistance, insulin-resistant diabetes mellitus, noninsulin-dependent diabetes mellitus (NIDDM), Rabson-Mendenhall syndrome and Leprechaunism (Donohue Syndrome).Synonyms: Insulin Receptor

Gene ID: 3643

NCBI Accession: NP_000199

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity