FOXP3 antibody (N-Term)

Catalog No. ABIN3026196

Product Details anti-FOXP3 Antibody

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

Clone: FHBP3-1

Isotype: IgG1 kappa

Application Details

Application Notes: Titering of the FOXP3 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the FOXP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3 (FOXP3 Products)

Synonyms: foxp3 antibody, FOXP3 antibody, AIID antibody, DIETER antibody, IPEX antibody, PIDX antibody, XPID antibody, JM2 antibody, scurfin antibody, sf antibody, RGD1562112 antibody, forkhead box P3 antibody, forkhead box P3a antibody, forkhead box P3 L homeolog antibody, FOXP3 antibody, foxp3a antibody, foxp3 antibody, foxp3.L antibody, Foxp3 antibody

Background: The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

Gene ID: 50943

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation