CD147 antibody

Catalog No. ABIN3026721

Product Details anti-CD147 Antibody

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Inhibition Assay (InhA)

Characteristics: This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Purification: Protein G purified CD147 antibody

Immunogen: Human T cell line Molt-13 was used as the immunogen for this CD147 antibody.

Clone: 8D6

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD147 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Functional Activity: Inhibits homotypic aggregation and adhesion to matrix proteins: order BSA-free antibody format,FACS: 0.5-1 μg/million cells,IF: 1:100-1:200,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CD147 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147 (BSG Products)

Synonyms: EMMPRIN antibody, RPE7 antibody, 5F7 antibody, CD147 antibody, M6 antibody, OK antibody, TCSF antibody, AI115436 antibody, AI325119 antibody, HT-7 antibody, 5A11 antibody, CE9 antibody, HT7 antibody, Ox47R antibody, basignin antibody, wu:fb26a05 antibody, wu:fo98e12 antibody, bsg antibody, prso03 antibody, basigin (Ok blood group) antibody, basigin antibody, basigin (Ok blood group) S homeolog antibody, BSG antibody, Bsg antibody, bsg antibody, bsg.S antibody

Background: This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

Gene ID: 682

Pathways: S100 Proteins