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- Target See all His Tag Antibodies
- His Tag
- Reactivity
- Please inquire
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This His Tag antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Sequence
- HHHHHH
- Purification
- Purified
- Immunogen
- Poly-His peptide was used as the immungen for the His Tag antibody.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product His Tag Primary Antibody
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anti-His Tag antibody
WB, ELISA, IP, IHC Host: Rabbit Polyclonal unconjugated
anti-His Tag antibodyReactivity: Human WB Host: Mouse Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA Host: Rabbit Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IHC, FACS Host: Mouse Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyReactivity: Tag, Human WB Host: Mouse Monoclonal 2D5 unconjugated
anti-His Tag antibody (HRP)WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 HRP
anti-His Tag antibodyReactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated
anti-His Tag (C-Term) antibodyWB, ELISA Host: Rabbit Polyclonal unconjugated
anti-His Tag antibody (Biotin)WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2 Biotin
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- Application Notes
- Titration of the His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot the His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- Target
- His Tag
- Abstract
- His Tag Products
- Target Type
- Tag
- Background
- Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.
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