AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (C-Term)

Details for Product anti-AIFM1 Antibody No. ABIN3029686
Target
Binding Specificity
C-Term
19
15
11
9
5
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Rat
124
72
71
24
14
13
9
9
8
7
5
4
4
2
1
1
Host
Rabbit
112
12
2
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
7
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
100
48
41
30
29
19
13
11
10
8
5
2
2
1
1
Options
Immunogen An amino acid sequence from the C-terminus of human AIFM1 (EQHEDLNEVAKLFNIHED) was used as the immunogen for this Apoptosis-Inducing Factor antibody (100% homologous in human, mouse and rat).
Isotype IgG
Purification Antigen affinity
Target
Alternative Name Apoptosis-Inducing Factor (AIFM1 Antibody Abstract)
Background Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.
UniProt O95831
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration
Application Notes The stated application concentrations are suggested starting amounts. Titration of the Apoptosis-Inducing Factor antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
Restrictions For Research Use only
Buffer 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage -20 °C
Storage Comment After reconstitution, the Apoptosis-Inducing Factor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Images
Image no. 1 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN3029686) IHC-P: Apoptosis-Inducing Factor antibody testing of human lung cancer tissue
Image no. 2 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN3029686) IHC-P: Apoptosis-Inducing Factor antibody testing of rat heart tissue
Image no. 3 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN3029686) Western blot testing of Apoptosis-Inducing Factor antibody and Lane 1: rat heart; 2...
Image no. 4 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN3029686) Western blot testing of Apoptosis-Inducing Factor antibody and Lane 1: rat heart
Image no. 5 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibody (ABIN3029686) IHC-P: Apoptosis-Inducing Factor antibody testing of rat heart tissue