BAX antibody (AA 19-33)

Catalog No. ABIN3030143

The Rabbit Polyclonal anti-BAX antibody is suitable to detect BAX in samples from Human, Mouse and Rat. It has been validated for WB, FACS and IHC (p).

Quick Overview for BAX antibody (AA 19-33) (ABIN3030143)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BAX Antibody

Binding Specificity: AA 19-33

Purpose: Bax Antibody

Sequence: IMKTGALLLQ GFIQD

Purification: Antigen affinity

Immunogen: Amino acids 19-33 (IMKTGALLLQGFIQD) were used as the immunogen for this Bax antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Bax antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Bax antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Background: Bcl-2 Associated X Protein is a member of the Bcl-2 gene family. The gene encodes a 21- kDa protein whose association with Bcl-2 is believed to play a critical role in regulating apoptosis. It is an apoptosis-inducing protein that participates in cell death during normal development and in various diseases. It resides in an inactive state in the cytosol of many cells. Bax consists of 9 alpha helices and has extensive amino acid homology with Bcl-2, focused within highly conserved domains I and II. The protein is encoded by six exons and demonstrates a complex pattern of alternative RNA splicing that predicts a 21 kd membrane (alpha) and two forms of cytosolic protein (beta and gamma). Bax and Bak are essential for regulating the number of B cells at both immature and mature developmental stages. They are critical mediators of B cell death induced by multiple stimuli.

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response