Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
Antigen affinity
Immunogen
An amino acid sequence from the middle region of human Disrupted in Schizophrenia 1 (EKQQLQKEIEALQARMF) was used as the immunogen for this DISC1 antibody.
The stated application concentrations are suggested starting amounts. Titration of the DISC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Mouse
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the DISC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
DISC1 antibody, C1orf136 antibody, SCZD9 antibody, disrupted in schizophrenia 1 antibody, disrupted in schizophrenia 1 protein antibody, disc1 antibody, DISC1 antibody, LOC100580606 antibody, Disc1 antibody
Background
Disrupted in Schizophrenia 1 is a protein that is encoded by the DISC1 gene in humans. Ma et al.(2002) determined that the mouse gene maps to chromosome 8 in a region with homology of synteny to human chromosome 1q42. Ozeki et al.(2003) demonstrated that rodent Disc1 expression displayed pronounced developmental regulation, with the highest levels in late embryonic life during development of the cerebral cortex. Millar et al.(2005) showed that the protein interacts with the UCR2 domain of phosphodiesterase-4B, implicated in susceptibility to schizophrenia, and that elevation of cellular cAMP leads to dissociation of PDE4B from DISC1 and in increase in PDE4B activity.