EIF3F antibody (AA 114-125)

Catalog No. ABIN303092

Product Details anti-EIF3F Antibody

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Binding Specificity: AA 114-125

Reactivity: Human, Mouse, Rat, Cow, Monkey, Chicken, Drosophila melanogaster, Fish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3F antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: Amino acids 114-125 of Human eIF3f protein.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Chimpanzee, Chicken, Drosophila, Fish , Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: KLH Synthetic peptide corresponding to amino acids 114-125 of Human eIF3f protein.

Application Details

Application Notes: Western Blot (1/200-1/2000). ELISA (1/10000-1/40000). Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This EIF3 F antibody was validated for use in Immunohistochemistry on a panel of 23 ormalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.

Target Details for EIF3F

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Alternative Name: EIF3F / EIF3S5 (EIF3F Products)

Synonyms: AO090005001590 antibody, Dmel\CG9769 antibody, eIF3-S5 antibody, eif3f antibody, LOC733116 antibody, eIF3f antibody, EIF3S5 antibody, eIF3-p47 antibody, 0610037M02Rik antibody, AA409853 antibody, Eif3s5 antibody, eukaryotic translation initiation factor 3 subunit F antibody, hypothetical protein antibody, CG9769 gene product from transcript CG9769-RA antibody, eukaryotic translation initiation factor 3 subunit 5 antibody, eukaryotic translation initiation factor 3, subunit F antibody, Eukaryotic translation initiation factor 3 subunit F antibody, eif3f antibody, ANI_1_120074 antibody, AOR_1_2780174 antibody, PTRG_03897 antibody, PAAG_00376 antibody, MCYG_08502 antibody, MGYG_08653 antibody, PGTG_04587 antibody, CG9769 antibody, LOC733116 antibody, EIF3F antibody, Eif3f antibody, eif-3.F antibody

Background: Eukaryotic translation initiation factor 3 subunit F also known as eIF3f, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.Synonyms: Eukaryotic translation initiation factor 3 subunit 5, Eukaryotic translation initiation factor 3 subunit F, eIF-3-epsilon, eIF3 p47

Gene ID: 8665

NCBI Accession: NP_003745

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization