Connexin 43/GJA1 antibody (AA 351-367)

Catalog No. ABIN3031019

The Rabbit Polyclonal anti-Connexin 43/GJA1 antibody has been validated for WB, IHC (p), ICC and IHC (fro). It is suitable to detect Connexin 43/GJA1 in samples from Human, Rat and Mouse.

Quick Overview for Connexin 43/GJA1 antibody (AA 351-367) (ABIN3031019)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: AA 351-367

Purification: Antigen affinity

Immunogen: Amino acids 351-367 (HELQPLAIVDQRPSSRA-human) were used as the immunogen for this Cx43 antibody (100% conserved in mouse and rat).

Isotype: IgG

Application Details

Application Notes: Titration of the Cx43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: The lyophilized Cx43 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Cx43 Connexin 43

Background: Connexin 43 (Cx43), also called GAP Junction Protein, alpha, is a member of the connexin gene family and is abundantly expressed in the heart and liver. Connexin 43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Cx43 also plays a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization