GLI2 antibody (C-Term)

Catalog No. ABIN3031040

Product Details anti-GLI2 Antibody

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human GLI-2 (MLYYYGQIHMYEQDGGLENL) was used as the immunogen for this GLI2 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Gli2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. GLI2 exists as five alternatively spliced isoforms of 133 kDa (isoform 1), 132 (2), 88 (3), 86 (4) and 168 kDa (isoform 5).\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the GLI2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2 (GLI2 Products)

Synonyms: HPE9 antibody, THP1 antibody, THP2 antibody, GLI2/GLI4 antibody, GLI3 antibody, AW546128 antibody, GLI family zinc finger 2 antibody, GLI-Kruppel family member GLI2 antibody, GLI2 antibody, Gli2 antibody

Background: Gli-Kruppel Family Member 2 is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel(Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes, thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al.(1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al.(2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

UniProt: P10070

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis