GFP antibody

Catalog No. ABIN3031105

This Mouse Monoclonal antibody specifically detects GFP in WB, ELISA and IF. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP antibody (ABIN3031105)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 168AT1211

Product Details anti-GFP Antibody

Purification: Purified

Immunogen: Purified His-tagged protein was used to produced this monoclonal YFP / GFP antibody.

Isotype: IgG1

Application Details

Application Notes: Titration of the GFP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:4000,Immunofluorescence: 1:50-1:100,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the GFP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Green fluorescent protein is one of the best visual reporters for monitoring gene expression in vivo and in situ. It is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. NSJBio's anti-GFP antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. NSJBio's anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.