MAP3K1 antibody (C-Term)

Catalog No. ABIN3031846

This Rabbit Polyclonal antibody specifically detects MAP3K1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MAP3K1 antibody (C-Term) (ABIN3031846)

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAP3K1 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human MEK kinase 1 (PEVLRGQQYGRSCDV) was used as the immunogen for this MAP3K1 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the MAP3K1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the MAP3K1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for MAP3K1

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Alternative Name: MAP3K1 (MEKK1)

Background: Mitogen-activated protein kinase kinase kinase 1, also known as MEKK1, MAPKKK1, MEK Kinase or MAP/ERK Kinase Kinase 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse gene and the human homolog. Using these allele-specific polymorphisms, they mapped the mouse gene in an intersubspecific backcross to chromosome 13. They mapped the human gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MAP3K1 / MEKK1 activated JNK1 (MAPK8) robustly and p38-alpha (MAPK14) less efficiently, but it had only a marginal effect on ERK2 (MAPK1). MAP3K1 / MEKK1 directly and specifically interacted with JNKK1 (MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.

UniProt: Q13233

Pathways: MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades