PAD4 antibody (Middle Region)

Catalog No. ABIN3032172

Product Details anti-PAD4 Antibody

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAD4 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human PAD4 (ASPRSCYKLFQEQQNEGHGEA) was used as the immunogen for this PAD4 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the PAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PAD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PAD4

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Alternative Name: PAD4 (PADI4 Products)

Synonyms: PAD antibody, PAD4 antibody, PADI5 antibody, PDI4 antibody, PDI5 antibody, Pad4 antibody, Pdi4 antibody, peptidyl arginine deiminase 4 antibody, peptidyl arginine deiminase, type IV antibody, PADI4 antibody, Padi4 antibody

Background: Peptidylarginine Deiminase, Type IV is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that the protein specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PAD4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PAD4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that the protein could repress hormone receptor-mediated gene induction. It was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role.

UniProt: Q9UM07