Prohibitin antibody (C-Term)

Catalog No. ABIN3032315

Product Details anti-Prohibitin Antibody

Target: Prohibitin (PHB)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prohibitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human PHB1 (KLEAAEDIAYQLSRSRN) was used as the immunogen for this Prohibitin antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Prohibitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Prohibitin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: Prohibitin (PHB Products)

Synonyms: phb1 antibody, MGC75944 antibody, PHB antibody, Phb antibody, ACYPI000080 antibody, phb antibody, PHB1 antibody, Bap32 antibody, sr:nyz128 antibody, wu:fa18f07 antibody, wu:fb09f05 antibody, prohibitin1 antibody, xphb1 antibody, prohibitin antibody, prohibitin-2 antibody, prohibitin L homeolog antibody, PHB antibody, phb antibody, LOC484431 antibody, LOC5576054 antibody, Phb antibody, phb.L antibody

Background: Prohibitin, also known as PHB1, is a protein that in humans is encoded by the PHB gene. White et al.(1991) mapped the gene to chromosome 17 by analysis of human-mouse somatic cell hybrid cell lines using a genomic fragment of human prohibitin DNA isolated from a library using the rat prohibitin cDNA clone. By in situ hybridization, they localized the gene to 17q21. Sato et al.(1992) isolated the human homolog of the rat gene and mapped it to 17q12-q21 by in situ hybridization. Proliferation of tumor cells depends on new blood vessel formation (angiogenesis) that accompanies malignant progression. Anti-cancer therapies using angiogenesis inhibitors or cytotoxic agents targeted to the vasculature of tumors have been evaluated in clinical trials. Although white fat is a nonmalignant tissue, it has the capability to quickly proliferate and expand. Furthermore, it is highly vascularized. Rupnick et al.(2002) showed that nonspecific angiogenesis inhibitors can prevent the development of obesity of mice.

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling