Integrin beta 2 antibody (Biotin)

Catalog No. ABIN303565

This Mouse Monoclonal antibody specifically detects Integrin beta 2 in FACS, WB, IP, Func and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Integrin beta 2 antibody (Biotin) (ABIN303565)

Target: Integrin beta 2 (ITGB2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-48

Product Details anti-Integrin beta 2 Antibody (Biotin)

Specificity: This antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin b2 subunit, b2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Purification: Protein-A affinity chromatography

Purity: > 95 % pure (by SDS-PAGE)

Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. Immunoprecipitation. Western blot: 2 μg/mL. Positive control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. Application note: Non-reducing conditions. SDS-PAGE (6 % separating gel). Immunohistochemistry on frozen sections. Functional application: The antibody MEM-48 induces high-affinity conformation of LFA-1 complex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 / ITGB2

Background: CD18, integrin b2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID: 3689

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation