Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Specificity
The mouse monoclonal antibody 6H6 recognizes CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.
Purification
Purified from cell culture supernatant by protein-A affinity chromatography
Immunogen
IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells.